Analysis Note

ControlTonsil, human skin tissue

Application

Research Sub CategoryIntegrins

Anti-Integrin β1 Antibody, clone P4G11 is an antibody against Integrin β1 for use in ELISA, FC, IC, IH, IH(P), IP, FUNC, CULT.

Research CategoryCell Structure

Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}

Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

Traditional formalin fixation is not recommended.

Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)

Immunoprecipitation: 5 µg/400 µL lysate (0.5% triton PBS plus Ca++)

FACS: 2-5µg per 10e6 cells, in the presence of Ca++.

Stimulation of cells: 2-10 µg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.

ELISA

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Human IL-1beta activated endothelial cells

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Protein A Purified immunoglobulin in 0.02M PBS, 0.25M NaCl, pH 7.6, with 0.1% NaN3.

Format: Purified

Specificity

Reacts with calcium-dependent epitopes on the integrin β1 subunit. When bound to β1, P4G11, activates β1 dependent binding to extra cellular matrix substrates.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.